HH-624毛细管柱 气相色谱法测定三氯蔗糖中的甲醇

名称：毛细管色谱柱

固定相：6% 氰丙基苯基／94% 二甲基聚硅氧烷
极性：弱极性到中等极性固定相
相当于 USP 固定相 G43

型号：HH-624

规格：30m*0.25mm*1.4um
应用：气相色谱法测定三氯蔗糖中的甲醇



浩瀚色谱（山东）应用技术开发有限公司建立测定三氯蔗糖中甲醇残留量的方法。方法：采用顶空毛细管气相色谱法。色谱柱为HH-624毛细管色谱柱，柱温采用程序升温（初始温度35℃，保持5 min，以50℃/min升温200℃，保持5 min），检测器为氢火焰离子化检测器。进样口温度为220℃，检测器温度为250℃，载气为氮气，流速为2.0 ml/min，分流比为10∶1，顶空温度为80℃，平衡时间为30 min，进样量为1 ml。结果：甲醇质量浓度在98.99～296.96μg/ml范围内与峰面积比值呈良好的线性关系（r＝0.9999）；精密度、稳定性、重复性试验的RSD均≤2.16％；平均加样回收率为100.20％，RSD为0.48％（n＝9）。三氯蔗糖低检测限为1.39μg/ml。3个厂家样品中有1个厂家的甲醇残留量为3.12μg/ml，另2个厂家的甲醇残留量低于低检测限。结论：该方法简便、快捷，适用于三氯蔗糖中甲醇的残留量测定。



Determination of Methanol in Sucralose by Gas Chromatography
Determination of Methanol in Sucralose by Gas Chromatography Details:
Name: Capillary Chromatographic Column

Stationary phase: 6% cyanopropyl phenyl/94% dimethyl polysiloxane
Polarity: weak to medium polarity stationary phase
Equivalent to USP stationary phase G43

Model: HH-624

Specification: 30m*0.25mm*1.4um
Application: Determination of methanol in sucralose by gas chromatography

Haohan Chromatography (Shandong) Applied Technology Development Co., Ltd. established a method for the determination of residual methanol in sucralose. Method: Use headspace capillary gas chromatography. The chromatographic column is an HH-624 capillary chromatographic column, and the column temperature adopts a program temperature increase (initial temperature 35℃, keep for 5 minutes, raise the temperature to 200℃ at 50℃/min, keep for 5 minutes), and the detector is a hydrogen flame ionization detector. The temperature of the injection port is 220°C, the temperature of the detector is 250°C, the carrier gas is nitrogen, the flow rate is 2.0 ml/min, the split ratio is 10:1, the headspace temperature is 80°C, the equilibrium time is 30 min, and the sample volume Is 1 ml. Results: The methanol mass concentration in the range of 98.99～296.96μg/ml has a good linear relationship with the peak area ratio (r=0.9999); the RSD of the precision, stability, and repeatability tests are all ≤2.16%; the average sample recovery rate It is 100.20%, and the RSD is 0.48% (n=9). The minimum detection limit of sucralose is 1.39μg/ml. Among the samples from the 3 manufacturers, the methanol residue of one manufacturer was 3.12μg/ml, and the methanol residue of the other two manufacturers was below the minimum detection limit. Conclusion: The method is simple, fast, and suitable for the determination of the residual methanol in sucralose.